WebNov 27, 2024 · D Cell lysates of P and GR PAN02 cells were subjected to Western blot analysis of ERK1/2, phosphorylated ERK1/2, AKT, phosphorylated AKT, and NF-kB. β-Actin was assayed as a loading control. E GR PAN02 cells were infected with OBP-301 or OBP-702 at the indicated MOIs for 72 h. Cell lysates were subjected to Western blot analysis … WebPan02 Growth Parameters Subcutaneous implant of Pan02 results in successful establishment of tumors that have a median doubling time of 4-5 days and mice stay on …
Oncolytic virus-mediated reducing of myeloid-derived suppressor cells ...
WebPan02 was derived from C57BL/6 mice given orthotopic 3-methyl-cholanthrene and is refractory to many standard chemotherapeutic agents. 1 Pan02 contains a loss of function mutation in the SMAD4 gene that is functionally similar to inactivating mutations in approximately 30% of human pancreatic cancers. 2 Mean and Individual Growth Curves WebFeb 14, 2012 · Pan02 cells have a mesenchymal-like phenotype. Fluorescence immunohistochemistry (A–B) and immunocytochemistry (C) were utilized to assess the epithelial versus mesenchymal nature of Pan02 cells. (A) Frozen tumor sections were stained with antibody rabbit anti E-Cadherin (red) to mark epithelial cells. DAPI (blue) … free printable award templates for kids
Pan02 Cells atcc Bioz
WebDec 8, 2016 · Here, we show that treatment of KPC and Pan02 murine PDAC cells with RT and gemcitabine upregulated PD-L1 expression in a JAK/Stat1-dependent manner. In … WebFeb 23, 2024 · PAN02was purchased from the National Infrastructure of Cell Line Resource (Beijing). All cells were cultured at 37 °C in a humidified incubator with 5% CO 2 in Dulbecco’s modified Eagle’s medium (DMEM) (HyClone) supplemented with 10% fetal bovine serum (FBS) (HyClone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco). WebOct 22, 2024 · Pan02 cells were incubated with 200 nM Cy5.5-conjugated atezolizumab, VEGF-Grab (negative control), or Ate-Grab for 6 h at 37 °C. After incubation, Pan02 cells were washed three times with PBS ... farmhouse french country decor