WebIn-Fusion® HD Cloning Kit User Manual . In-Fusion® HD Cloning Kit User Manual WebApr 12, 2013 · Control Insert 4-10-13 (12.3 ng/uL) Control Vector 4-10-13 (10.6 ng/uL) Control insert and vector Insert size (2 kb Control Insert): 2 kb Linearized Vector size (pUC19 Control Vector, linearized): 2686 bp I will use In-Fusion molar ratio calculator should use 100 ng vector 148.9 ng insert I could reduce these by 4 if desired 25 ng vector 37.2 ...
Differential Transformation Efficiencies Observed for pUC19 and …
WebA “stuffer” sequence allows for electrophoretic separation of linearized vector from the reaction ... 10-50 ng/μl: 1: dGTP (100mM) 2.5 mM: 2: DTT (100 mM) 5 mM: 1: BSA (10 … Web500 bp control insert (20 ng/μl) 5 μl. 5 μl. pUC19 control vector，linearized (50 ng/μl，Amp+ ... covid 19 impfung apotheke
SAFETY DATA SHEET - NEB
WebSalt Solution 1.2 M NaCl, 0.06 M MgCl2 50 μL Sterile Water — 1 mL Control PCR template 50 ng/μL in TE buffer[1], pH 8.0 10 μL Control PCR primers 100 ng/μL each in TE buffer, pH 8.0 10 μL Polyhedrin forward sequencing primer 100 ng/μL in TE buffer, pH 8.0 20 μL SV40 polyA reverse sequencing primer 100 ng/μL in TE buffer, pH 8.0 20 μL http://www.microbiology.ubc.ca/sites/default/files/roles/drupal_ungrad/JEMI/8/8-8.pdf WebpUC19 control vector, linearized (50ng/μl) 5μl 5 μl×5 运输与保存方法 冰袋（wet ice）运输。产品-20 ºC保存。 实验流程 实验流程概览 1)线性化克隆载体制备； 2)插入片段扩增引物设计； 3)插入片段PCR扩增； 4)进行重组反应； 5)反应产物转化、涂板； 4.重组产物转化、涂板 covid 19 impfung und sport